![]() ![]() If you want to measure the concentration of the final solution after two successive 10-fold dilutions, follow the same formula: (6 M X1/10 X 1/10) = 6/100 = 0.06 M In other words, your sample has been diluted 100-fold (d.f. Your final dilution ends up being 1/10 X 1/10 = 1/100 dilution. Let’s say you again diluted the sugar solution by taking 1 ml of diluted sugar solution in another 9 mL of water. The diluent may be water, a buffer solution, or an actual growth medium. In a real-world example, the sample can be an antibiotic solution, a patient specimen, food items, or drinking water. In this example, we have used sugar solution as a sample and water as a diluent. Undiluted concentration = (Diluted concentration X dilution factor) Let’s say you have a sample that has been diluted 1/10 with a concentration of 0.6 M and wants to calculate its undiluted concentration. Sometimes, you may wish to calculate backward. To calculate the concentration of this diluted sample, multiply by the inverse of the dilution factor.įinal concentration = (Initial concentration X 1/ dilution factor), i.e., 0.6 M The dilution factor is 10 (dilution factor is the reciprocal of the dilution).This dilution can be expressed by various terms like Total volume = (Volume of sample + Volume of diluent), i.e., 1 mL sugar solution + 9 mL water = 10 mLĭilution Factor = Total volume/Sample volume = 10/1= 10 To calculate the dilution factor (df), you need to divide the solution’s total volume by the sample volume (i.e., sugar solution in this case). If you took 1 mL of sugar solution and dispensed it in a new tube, then added 9 mL of water and mixed. Let’s say you have a glass of sugar solution at 6.0 M concentration and another glass with water. If you are already familiar with the basics, you can directly jump to preparing serial dilution). (In this section, I am discussing very basic information about dilution and terminologies. Advantages of Serial Dilution Understanding Basics of Dilution.To obtain a pure culture of microorganisms. ![]()
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